Part I The Basic Principles of Gene Cloning and DNA Analysis 1

  1.  Why Gene Cloning and DNA Analysis are Important 3

  2.  Vectors for Gene Cloning: Plasmids and Bacteriophages 15

  3.  Purification of DNA from Living Cells 29

  4.  Manipulation of Purified DNA 53

  5.  Introduction of DNA into Living Cells 83

  6.  Cloning Vectors for E. coli 101

  7.  Cloning Vectors for Eukaryotes 121

  8.  How to Obtain a Clone of a Specific Gene 145

  9.  The Polymerase Chain Reaction 169

Part II The Applications of Gene Cloning and DNA Analysis in Research 187

10.  Sequencing Genes and Genomes 189

11.  Studying Gene Expression and Function 217

12.  Studying Genomes 243

13.  Studying Transcriptomes and Proteomes 259

Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 275

14.  Production of Protein from Cloned Genes 277

15.  Gene Cloning and DNA Analysis in Medicine 301

16.  Gene Cloning and DNA Analysis in Agriculture 327

17.  Gene Cloning and DNA Analysis in Forensic Science and Archaeology 355

Glossary 377

Index 395