1 Why Gene Cloning and DNA Analysis are Important 3

2 Vectors for Gene Cloning: Plasmids and Bacteriophages 13

3 Purification of DNA from Living Cells 25

4 Manipulation of Purified DNA 47

5 Introduction of DNA into Living Cells 75

6 Cloning Vectors for Escherichia coli 93

7 Cloning Vectors for Eukaryotes 111

8 How to Obtain a Clone of a Specific Gene 135

9 The Polymerase Chain Reaction 157

Part II The Applications of Gene Cloning and DNA Analysis in Research 173

10 Sequencing Genes and Genomes 175

11 Studying Gene Expression and Function 201

12 Studying Genomes 225

Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 245

13 Production of Protein from Cloned Genes 247

14 Gene Cloning and DNA Analysis in Medicine 269

15 Gene Cloning and DNA Analysis in Agriculture 291

16 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 311

Glossary 329

Index 345