An Introduction to Molecular Biotechnology: Fundamentals, Methods and Applications 3/e 2021
FUNDAMENTALS OF CELLULAR AND MOLECULAR BIOLOGY The Cell as the Basic Unit of Life Structure and Function of Cellular Macromolecules Structure and Functions of a Cell Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins) Distributing Proteins in the Cell (Protein Sorting) Evolution and Diversity of Organisms STANDARD METHODS IN MOLECULAR BIOTECHNOLOGY Isolation and Purification of Proteins Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry Isolation of DNA and RNA Chromatography and Electrophoresis of Nucleic Acids Hybridization of Nucleic Acids Use of Enzymes in the Modification of Nucleic Acids Polymerase Chain Reaction DNA Sequencing Genome Editing Cloning Procedures Expression of Recombinant Proteins Omics technologies Patch Clamp Method Cell Cycle Analysis Microscopic Techniques Laser Applications KEY TOPICS Genomics and Functional Genomics Bioinformatics Cellular Systems Biology Protein-Protein and Protein-DNA Interaction Drug Research Drug Targeting and Prodrugs Molecular Diagnostics and Personalized Medicine Recombinant Antibodies and Phage Display Transgenic and Gene-Targeted Mice Plant Biotechnology Industrial Biocatalysis BIOTECHNOLOGY IN INDUSTRY The Biotech Industry, Markets, and Opportunities Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues Drug Approval in the European Union and United States Founding a Biotech Company APPENDIX Further Reading Abbreviations Subject Index