An Introduction to Molecular Biotechnology: Fundamentals, Methods and Applications 3/e 2021
目次

Part I Fundamentals of Cellular and Molecular Biology

1 The Cell as the Basic Unit of Life

2 Structure and Function of Cellular Macromolecules

3 Structure and Functions of a Cell

4 Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins)

5 Distributing Proteins in the Cell (Protein Sorting)

6 Evolution and Diversity of Organisms

Part II Standard Methods in Molecular Biotechnology

7 Isolation and Purification of Proteins

8 Mass Spectrometry and Applications in Proteomics and Microbial Identification

9 Isolation of DNA and RNA

10 Chromatography and Electrophoresis of Nucleic Acids

11 Hybridization of Nucleic Acids

12 Use of Enzymes in the Modification of Nucleic Acids

13 Polymerase Chain Reaction

14 DNA Sequencing

15 Cloning Procedures

16 Expression of Recombinant Proteins

17 Patch Clamp Method

18 Cell Cycle Analysis

19 Microscopic Techniques

20 Laser Applications

Part III Key Topics

21 Sequencing the Universe of Life

22 Cellular Systems Biology

23 Protein–Protein and Protein–DNA Interactions

24 Bioinformatics

25 Drug Research

26 Drug Targeting and Prodrugs

27 Molecular Diagnostics in Medicine

28 Recombinant Antibodies and Phage Display

29 Genetically Modified Mice and Their Impact in Medical Research

30 Plant Biotechnology

31 Biocatalysis in the Chemical Industry

Part IV Biotechnology in Industry

32 Industrial Application: Biotech Industry,Markets, and Opportunities

33 Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues

34 Drug Approval in the European Union and United States

35 Emergence of a Biotechnology Industry

36 The 101 of Founding a Biotech Company

37 Marketing


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