內容簡介

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

作者簡介

◎Keith Wilson is Professor Emeritus of Pharmacological Biochemistry and former Head of the Department of Biosciences, Dean of the Faculty of Natural Sciences, and Director of Research at the University of Hertfordshire.

◎John Walker is Professor Emeritus and former Head of the School of Life Sciences at the University of Hertfordshire.

目次

1. General principles K. Wilson
2. Cell culture techniques A. R. Baydoun
3. Centrifugation K. Ohlendieck
4. Microscopy S. W. Paddock
5. Molecular biology, bioformatics and basic techniques R. Rapley
6. Recombinant DNA and genetic analysis R. Rapley
7. Immunological techniques R. Burns
8. Protein structure, purification, characterisation and functional analysis J. M. Walker
9. Mass spectrometric techniques A. Aitken
10. Electrophoretic techniques J. Walker
11. Chromatographic techniques K. Wilson
12. Spectroscopic techniques I A. Hofmann
13. Spectroscopic techniques II A. Hofmann
14. Radioisotope techniques R. Slater
15. Enzymes K. Wilson
16. Principles of clinical biochemistry J. Fyffe and K. Wilson
17. Cell membrane receptors K. Wilson
18. Drug discovery and development K. Wilson.