Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.

 

This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.

 

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.

1.1 Historical Background

1.2 Advantages of Tissue Culture

1.2.1 Control of the Environment

1.2.2 Characterization and Homogeneity of Sample

1.2.3 Economy, Scale, and Mechanization

1.2.4 In vitro Modeling of In vivo Conditions

1.3 Limitations

1.3.1 Expertise

1.3.2 Quantity

1.3.3 Dedifferentiation and Selection 1.3.4 Origin of Cells

1.3.5 Instability

1.4 Major Differences In Vitro

1.5 Types of Tissue Culture

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2.1 The Culture Environment

2.2 Cell Adhesion

2.2.1 Cell Adhesion Molecules

2.2.2 Intercellular Junctions

2.2.3 Cytoskeleton

2.2.4 Extracellular Matrix

2.2.5 Cell Motility

2.3 Cell Proliferation

2.3.1 Cell Cycle

2.3.2 Control of Cell Proliferation

2.4 Differentiation

2.4.1 Induction and Maintenance of Differentiation

2.4.2 Plasticity of Differentiation and Dedifferentiation

2.5 Cell Signaling 2.6 Energy Metabolism

2.7 Origin of Cultured Cells

2.7.1 Initiation of the Culture

2.7.2 Evolution of Cell Lines

2.7.3 Senescence

2.7.4 Transformation and the Development of Continuous Cell Lines

2.8 Definitions

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3.1 Planning, Furnishing, and Services

3.1.1 Requirements

3.1.2 Services

3.1.3 Ventilation

3.2 Design and Layout

3.2.1 Sterile Handling Area

3.2.2 Laminar Flow Hoods

3.2.3 Service Bench

3.2.4 Quarantine and Containment

3.2.5 Incubation

3.2.6 Preparation Area

3.2.7 Storage 3.3 Disaster Management

References

4.1 Requirements of a Tissue Culture Laboratory

4.2 Sterile Working Area

4.2.1 Laminar-Flow BSC

4.2.2 Service Carts

4.2.3 Sterile Liquid Handling-Pipetting and Dispensing

4.2.4 Inverted Microscope

4.2.5 Camera and Monitor

4.2.6 Dissecting Microscope

4.2.7 Centrifuge

4.2.8 Cell Counting

4.3 Incubation and Culture

4.3.1 Incubator

4.3.2 Humid CO2 Incubator

4.3.3 Temperature Recorders

4.3.4 Roller Racks

4.3.5 Magnetic Stirrer

4.3.6 Culture Vessels

4.4 Preparation and Sterilization 4.4.1 Washup

4.4.2 Preparation of Media and Reagents

4.4.3 Sterilization

4.5 Storage

4.5.1 Consumables

4.5.2 Refrigerators and Freezers

4.5.3 Cryostorage Containers

4.5.4 Controlled-Rate Freezer

4.6 Supplementary Laboratory Equipment

4.6.1 Computers and Networks

4.6.2 Upright Microscope

4.6.3 Low-Temperature Freezer

4.6.4 Confocal Microscope

4.6.5 PCR Thermal Cycler

4.7 Specialized Equipment

4.7.1 Microinjection Facilities

4.7.2 Colony Counter

4.7.3 Centrifugal Elutriator

4.7.4 Flow Cytometer

References

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