專業研究用書,無法提供教師樣書
內容簡介
This is a comprehensive and cutting-edge collection of basic and advanced biochemical techniques for isolating, treating, and analyzing the photosynthetic materials and processes. All protocols follow the successful "Methods in Molecular Biologya" series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
目次
Fractionation of thylakoid membranes into grana and stroma thylakoids / Juan Cuello and Maria Jose Quiles
Isolation of photosystem I particles from spinach / Tetsuo Hiyama
Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii / Velupillai M. Ramesh and Andrew N. Webber
Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants / Yasusi Yamamoto, Shinsuke Sakuma, and Jian-Ren Shen
Isolation of functional photosystem II core particles from the Cyanobacterium synechocystis sp. PCC 6803 / Dmitrii Vavilin
Isolation of photosystem I reaction center preparation from spinach / Tetsuo Hiyama
Isolation of photosystem II reaction center complexes from plants
Michael Seibert, Inmaculada Yruela, and Rafael Picorel
Isolation of photosystem I reaction center subunit polypeptide from spinach / Tetsuo Hiyama. Purification of the cytochrome b6f complex in oxygenic photosynthesis / Huamin Zhang and William A. Cramer
Purification of plastocyanin and cytochrome c6 from plants, green algae, and cyanobacteria / Jose A. Navarro, Manuel Hervçs, and Miguel A. De la Rosa
Preparation of native and recombinant light-harvesting chlorophyll-a/b complex / Wolfgang Rohle and Harald Paulsen
Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macroassemblies with light-inducible structural transitions / Ilian Simidjiev, Zsuzsanna Vçrkonyi, and GyÜzÜ Garab
Separation, purification and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII) / Grzegorz Jackowski
Isolation of CP43 and CP47 photosystem II proximal antenna complexes for plants / Rafael Picorel, Miguel Alfonso, and Michael Seibert. The determination and quantification of photosynthetic pigments by reverse phase high performance liquid chromatography (HPLC), thin layer chromatography, and spectroscopy / Tessa Pocock, Marianna Krùl, and Norman P.A. Huner
Isolation and identification of chloroplast lipids / Sato and Mikio Tsuzuki
DNA adducts with chlorophyll and chlorophyllin as antimutagenic agents: synthesis, stability, and structural features / Heidar-Ali Tajmir-Riahi, Jean-Francois Neault, and Stravoula Diamantoglou
Incorporation and analysis of LHCII in model systems / Wieslaw I. Gruszecki
Photosystem II reconstitution into proteoliposomes: structure-function characterization / Mario Fragata
Extraction of the functional manganese and calcium cofactors from photosystem II / Joel Freeman, Garth Hendry, and Tom Wydrzynski
Assay of photoinhibition of photosystem II and protease activity / Yasusi Yamamoto, Yoji Nishi, Hitoshi Yamasaki, Suguru Uchida, and Satoshi Ohira. Thermoluminescence: a technique for probing photosystem II / Prafullachandra V. Sane
Detection of free radicals and reactive oxygen species / Eva Hideg
Stabilization of photosynthetic materials / Regis Rouillon, Pierre Euzet, and Robert Carpentier
Determination of phosphoproteins in higher plant thylakoids / Eva-Mari Aro, Anne Rokka, and Alexander V. Vener
Identifying photoprotection mutants in Arabidopsis thaliana / Jan Bart Rossel, Abby Cuttriss, and Barry J. Pogson
A simple method for chloroplast transformation in Chlamydomonas reinhardtii / Velupillai M. Ramesh, Scott E. Bingham, and Andrew N. Webber
Construction of gene knockouts in the Cyanobacterium synechocystis pcc 6803 / Julian J. Eaton-Rye
Gene inactivation in the cyanobacterium synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation / Niels-Ulrik Frigaard, Yumiko Sakuragi, and Donald A. Bryant
ISBN-13碼 :
9781588292322
ISBN-10碼 :
1588292320
出版日期 :
2004
書系代碼 :
HP015
作者 :
Robert Carpentier
開數 :
16開
頁數 :
344
裝訂 :
精裝
印刷 :
黑白
定價 :
3485