For both volumes:

 

Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

BAC library construction / K. Osoegawa and P.J. de Jong

Construction of small genome BAC library for functional and genomic applications / D. Smajs, S.J. Norris and G.M. Weinstock

Preparation of BAC libraries from bacterial genomes by in vitro packaging / S. Pal, S. Mak and G.M. Weinstock

Exploring transformation-associated recombination cloning for selective isolation of genomic regions / N. Kouprina [and others]

Purification of BAC DNA / T.S. Poulsen

Hybridization-based selection of BAC clones / C.S. Lim and U.J. Kim

Applications of interspersed repeat sequence polymerase chain reaction / H. Zimdahl [and others]

BAC mapping using fluorescence in situ hybridization / X.N. Chen and J.R. Korenberg

High-throughput BAC fingerprinting / J. Schein [and others]

BAC end sequencing / T.S. Poulsen and H.E. Johnsen

Radiation hybrid mapping with BAC ends / M. Olivier, S. Brady and D.R. Cox

Shotgun library construction for DNA sequencing / B.A. Roe

Rolling circle amplification for sequencing templates / P.F. Predki [and others]

Transposon-mediated sequencing / R. Reeg and A. Madan

Pyrosequencing: A tool for DNA sequencing analysis / E. Elahi and M. Ronaghi

Use of fimers to eliminate polymerase chain reaction and primer-dimer artifacts and to increase yield in bac-sequencing reactions / A. Malykh [and others]

Retrofitting bacs and pacs with loxP transposons to generate nested deletions / P.K. Chatterjee

Preparing nested-deletion template DNA for field inversion gel electrophoresis analyses and position-specific end sequencing with transposon primers / P.K. Chatterjee and J.C. Baker, Jr

BAC finishing strategies / C. Bird and D. Grafham

Using the TIGR assembler in shotgun sequencing projects / M. Pop and D. Kosack

Finishing "working draft" BAC projects by directed sequencing with thermofidelase and fimers / A. Malykh [and others]

Optimized multiplex polymerase chain reaction: An effective method for rapid gap closure / D. Radune and H. Tettelin

Assembly of DNA sequencing data / J. Schmutz, J. Grimwood and R.M. Myers

Sequence finishing / J. Schmutz, J. Grimwood and R.M. Myers

Quality assessment of finished BAC sequences / J. Schmutz, J. Grimwood and R.M. Myers