專業研究用書，無法提供教師樣書
內容簡介
Internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing mtDNA and illuminating the role it plays in the aging process, apoptosis, and possibly some cancers.
目次
pt. 1. Methods for the analysis of mtDNA
Animal models for mitochondrial disease / Douglas C. Wallace
Identification of mutations in mtDNA from patients suffering mitochondrial diseases / Eric A. Schon
Screening for aging-dependent point mutations in mtDNA / Yuichi Michikawa
Scanning low-frequency point mutants in the mitochondrial genome using constant denaturant capillary electrophoresis / Weiming Zheng
Identification of mtDNA mutations in human cancer / Shuji Nomoto
In situ PCR of the common human mt DNA deletion: is it related to apoptosis? / Steven J Zullo
Reproducible quantitative PCR of mitochondrial and nuclear DNA copy number using the LightCycler / Alice Wong
Yeast nuclear genes for mtDNA maintenance / Francoise Foury
Measuring mtDNA mutation rates in Saccharomyces cerevisiae using the mtArg8 assay / Micheline K. Strand
Measuring oxidative mtDNA damage and repair using quantitative PCR / Janine H. Santos
Migration of mtDNA into the nucleus / Mary K. Thorsness
2D gel electrophoresis of mtDNA / Heather E. Lorimer
pt. 2. Methods for the analysis of proteins involved in mtDNA transactions
Purifications of mitochondria for enzymes involved in nucleic acid transactions / Daniel F. Bogenhagen
Purification of mitochondrial uracil-DNA glycosylase using Ugi-sepharose affinity chromatography / Samuel E. Bennett
Characterization of specialized mtDNA glycosylases / Simon G. Nyaga
Purification, separation, and identification of the human mtDNA polymerase with and without its accessory subunit / Matthew J. Longley
Assay of mtDNA polymerase y from human tissues / Robert K. Naviaux
Purification strategies for Drosophila mtDNA replication proteins in native and recombinant form: DNA polymerase y / Carol L. Farr
Purification strategies for Drosophila mtDNA replication proteins in native and recombinant form: mitochondrial single-stranded DNA-binding protein / Carol L. Farr
Expression, purification, and in vitro assays of mitochondrial single-stranded DNA-binding protein / Kang Li
Recombinant yeast mtDNA helicases: purification and functional assays / Silja Kuusk
Isolation of DNA topoisomerases I and II activities from mitochondria of mammalian heart / Robert L. Low
Endonuclease G isolation and assays / Robert L. Low
Targeting DNA repair proteins to mitochondria / Allison W. Dobson
In organello footprinting of mtDNA / Steven C. Ghivizzani
Crosslinking of proteins to mtDNA / Brett A. Kaufman
Breeding and genotyping ot Tfam conditional knockout mice / Mats Ekstrand
