PCR Detection of Microbial Pathogens 2003

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內容簡介

Hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.

PART I REVIEWS

1 Specificity and Performance of Diagnostic PCR Assays

Konrad Sachse

2 Pre-PCR Processing of Samples

Peter Rådström, Rickard Knutsson, Petra Wolffs, Maria Dahlenborg, and Charlotta Lofstrom

3 Critical Aspects of Standardization of PCR

Jeffrey Hoorfar and Nigel Cook

4 PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens

Peter Stephensen Lübeck and Jeffrey Hoorfar

PART II PROTOCOLS

5 Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae

Joachim Frey

6 Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR

Darla R. Ewalt and Betsy J. Bricker

7 Isolation of Campylobacter and Identification by PCR

Mark D. Englen, Scott R. Ladely, and Paula J. Fedorka-Cray

8 Detection and Differentiation of Chlamydiae by Nested PCR

Konrad Sachse and Helmut Hotzel

9 Detection of Toxigenic Clostridia

Michel R. Popoff

10 PCR-Based Detection of Coxiella burnetii from Clinical Samples

Mustapha Berri, Nathalie Arricau-Bouvery, and Annie Rodolakis

11 Detection and Subtyping of Shiga Toxin-Producing Escherichia coli (STEC)

Peter Gallien

12 Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay

Louise O'Connor

13 Detection of Leptospira interrogans

John W. Lester and Rance B. LeFebvre

14 Detection of Pathogenic Mycobacteria of Veterinary Importance

Robin A. Skuce, M. Siobhan Hughes, Malcolm J. Taylor, and Sydney D. Neill

15 Multiplex PCR of Avian Pathogenic Mycoplasmas

Mazhar I. Khan

16 Detection and Differentiation of Ruminant Mycoplasmas

Helmut Hotzel, Joachim Frey, John Bashiruddin, and Konrad Sachse

17 Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air

Marylene Kobisch and Joachim Frey

18 PCR-Detection of Hemophilus paragallinarum, Hemophilus somnus,

Mannheimia (Pasteurella) hemolytica, Mannheimia spp.,

Pasteurella trehalosi, and Pasteurella multocida

Henrik Christensen, Magne Bisgaard, Jesper Larsen, and John Elmerdahl Olsen

19 Detection of Salmonella spp.

Burkhard Malorny and Reiner Helmuth

20 Detection of Toxoplasma gondii

Jonathan M. Wastling and Jens G. Mattsson

21 PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples

Edoardo Pozio and Giuseppe La Rosa

22 Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure

Rickard Knutsson and Peter Rådström

Index

ISBN-13碼 : 9781588290496

ISBN-10碼 : 1588290492

出版日期 : 2003

書系代碼 : HP025

作者 : Konrad Sachse．Joachim Frey

開數 : 16開

頁數 : 334

裝訂 : 精裝

印刷 : 黑白

定價 : 3485