Microwave Techniques and Protocols 2001

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Microwave Techniques and Protocols 2001

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內容簡介

The contributors to this manual are uniformly from those labora- ries routinely using microwave technology to facilitate their processing methods in the various fields of microscopy. The methods and results these authors describe are the tangible evidence that microwaves can be used routinely as the basis for improved sample processing for micr- copy applications. These applications include complete sample proce- ing protocols for light and electron microscopy, decalcification, and immunocytochemistry. The overall time savings, ease of use, and qu- ity of results serve as justification for using microwaves in the labo- tory. The question as to whether there is a "microwave effect" is alluded to, but not discussed in any great detail. When the term microwave technology is used, it is generic and intended to mean equipment designed for laboratory versus household use. Microwave Techniques and Protocols is designed for anyone with a background and experience in sample processing for immunocytoch- istry, decalcification, light microscopy, or electron microscopy, and clearly demonstrates that microwave technology has a place in today's laboratory. Richard T. Giberson, Richard S. Demaree Jr., REFERENCES Giberson, R. T. Demaree, R. S., and (1995) Microwave fixation: understa- ing the variables to achieve rapid reproducible results. Microsc Res Tech 32:246-254. Giberson, R. T., Demaree, R. S., Jr., and Nordhausen, R. W. (1997) Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J Vet Diagn Invest 9:61-67. Jr. VhD Ms vi Preface


目次

Overview of Microwave-Assisted Tissue Processing for Transmission Electron Microscopy
Vacuum-Assisted Microwave Processing of Animal Tissues for Electron Microscopy
Vacuum-Microwave Combination for Processing Plant Tissues for Electron Microscopy
Basic Procedure for Electron Microscopy Processing and Staining in Clinical Laboratory Using Microwave Oven
Specimen Preparation for Thin-Section Electron Microscopy Utilizing Microwave-Assisted Rapid Processing in a Veterinary Diagnostic Laboratory
Microwave Processing of Archived Pathology Specimens for Ultrastructural Examination
Microwave Fixation of Rat Hippocampal Slices
Microwave Processing Techniques for Biological Samples in a Service Laboratory
Microwave-Accelerated Decalcification
Microwave Processing of Sediment Samples
Microwave Polymerization in Thin Layers of London Resin White Allows Selection of Specimens for Immunogold Labeling
In Vivo Microwave-Assisted Labeling of Allium and Drosophila Nuclei
Microwave-Assisted Cytochemistry
Microwave-Assisted Immunoelectron Microscopy of Skin
Microwave Paraffin Techniques for Botanical Tissues
Microwave-Assisted Formalin Fixation of Fresh Tissue
Microwave-Assisted Processing of Biological Samples for Scanning Electron Microscopy

ISBN-13碼 : 9780896039032
ISBN-10碼 : 089603903X
出版日期 : 2001
書系代碼 : HP026
作者 : Richard T. Giberson.Richard S. Demaree Jr.
開數 : 16開
頁數 : 219
裝訂 : 平裝
印刷 : 黑白
定價 : 4775

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